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Ellenberger and co-personnel (eight) had intuited specifically such a motion with the OB area when they solved the crystal construction in the much larger human DNA ligase I (HuLig1; 919 amino acids) certain to an adenylylated nick. Their landmark construction was the 1st to doc the circumferential envelopment of your DNA duplex by a ligase clamp and the ensuing bending and distortion of your duplex that drive The bottom pairs about the 3′-OH facet of your nick into an บริการเดิมพันบอล RNA-just like a helical conformation (eight). Very similar distortions were noticed within the ChVLig·DNA elaborate (seven).
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